The plasmid ColV in the Adherence of Escherichia coli.

Adherence is an important mechanism of bacterial pathogenicity however, factors involved in the expression of septicemic infection caused by E. coli, are not completely understood. We studied the role of the ColV plasmid in the expression of adhesins in E.coli, for that purpose we compared the strain E. coli YA21 ColV+ isolated from colisepticemic poultry, with its isogenic E. coli YA21 ColV-, in addition E. coli K12 711 was compared with its isogenic E. coli K12 711 ColV+ obtained by conjugation with the strain the hability of glass adhesion was compared. Bacteria were harvested in logaritmic phase, then were adjusted to 4x 107/ml and incubated in RPMI at 41°C, subsecuently the number of adhesive bacteria were determinated in 10 fields by cuadruplicated.

Results:
We found highly significative diference in the glalss adherence of E. coli in the ColV+ strais when they were compared with their isogenics ColV- (p<0.0001). Similar results were obtained when bacterial adherence was assesed in HEp-2 cells.
Our results suggest that plasmid ColV mediate the expression of adhesins in E coli.

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